How can we calculate reliable significant changes for all group members?

Hello dear all, To calculate clinically significant changes (RC) in experimental study according to the formula from Jacobson and Truax (1991), what numbers should be placed for the all group...

06 September 2020 749 1 View

Flowcytometry after chemotaxis assay?

I did flowcytometry on the migrated t cells in chemotaxis assay to assess some surface markers. However the number of events is pretty low (less than 4000, aurora machine, high flow rate), which...

27 August 2020 1,023 3 View

How can we define artificial intelligence, in biotechnology and chemical engineering projects?

How can we define artificial intelligence, in biotechnology and chemical engineering projects? I want to combine these two areas But I have no background in artificial intelligence

26 March 2020 5,370 1 View

How is Golgi staining procedure for Drosophila brain?

I look for a robust and simple protocol for Golgi staining of Drosophila CNS. Please share your experience in this matter with me.

01 October 2019 9,029 1 View

Why it is hard to grow graphen on copper ?

and why it is spining and don't sit on copper easily? which crystal charastiric caused that?

19 February 2018 5,069 3 View

What are the best ways to write and edit an article?

1. Read articles in prestigious journals

01 October 2017 486 2 View

8-12 qubit graph States?

28 August 2015 3,516 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Useful architecture for bioinformatics?

Hi Dear researchers In ensemble-based architectural design Which algorithms are more useful for classification? What is the difference between parallel and ensemble architecture? Thanks I am...

08 February 2021 2,727 5 View

Electron density difference maps(Fo-Fc): what is the meaning of negative densities (red blobs)?

Dear community, As far as I understand, negative density features point at stuff that is in the model, but not supported by experimental data. However, in many cases (e.g. attached image), when...

06 February 2021 2,847 2 View

Qiime2- No reads passed the filter. How to set the trunc value?

Hi, I am working on some 16S sequencing data, and seems some of them are low quality. I am not sure how to set the trunc value in dada2. qiime dada2 denoise-paired \ --i-demultiplexed-seqs...

30 January 2021 6,213 3 View

Which software to use to statistically analyze Telomere Length_TRF_Southern Blot?

I am trying to measure telomere length in an ant species using the TRF method, which is a Southern blot technique. Currently, I am struggling with analyzing the images and would appreciate any...

29 January 2021 604 3 View

MHC Rabbit Genome Annotation? Domain annotation of each MHC Rabbit Gene?

I want to find a database(s) or a method where I can find and bulk download all the rabbit MHC genes and domain annotation. In humans using Ensembl, all MHC genes have names and domains but in...

28 January 2021 7,722 2 View

Best tool to build missing residues, minimize and validate a ligand model?

I am working on a ligand that is co-crystalized to parotein, but the ligand is missing some residues that makes it appear as if it was separated into two ligands! what I need is to connect them...

23 January 2021 3,975 7 View

What's the role of bioinformatics in poultry science?

bioinformatics has a big importance in human science and lots are seeking to study that field, so it attracts me to know it's relation with poultry science and how we can benefit from it in...

20 January 2021 6,963 3 View

How can I get the chromosomal location of a list of genes?

I'm looking for a bioinformatics website to get the chromosomal location of a radish gene id list, but in one shot instead of doing it gene by gene. Is there a way to do this? Thanks

17 January 2021 8,810 3 View