I have never extracted RNA from Mycobacteria and want to run an RNA gel, just to see how different extraction methods affect the RNA.
Any ideas would be appreciated.
Thank you.
the tablet should be compressed n the mixture of paste shouldnt be heated much
16 October 2023 9,718 1 View
Hello eveyone, I am trying to use nsolve of sympy to find solutions of system of non-linear algebraic equations. My system has three non-linear equations with three unknowns. Each of these...
01 March 2023 4,481 0 View
I want to find out degradation rate of PLGA formulation w.r.t. time for which change in initial molecular weight would be beneficial. Moreover, I don't have GPC facility. Please help me if anyone...
11 November 2021 3,717 1 View
I used the following command: movestay (lnTotalincome = HHsize h1sex TLU yrcop tland tprod) (ccert = HHsize h1sex TLU yrcop tland tprod), select (train = h1edu) But this doesnot work and I get...
01 November 2020 1,367 2 View
Hello every one, I am doing analysis of Bridge in which elastomeric bearing is modeled as link element between Deck and pier bent. Pier bent portal consist 2 pier and one cap beam on which...
12 August 2020 8,826 2 View
I am going to conduct the field survey to collect the major soil living organisms, beneficial insects, natural enemies, and decomposers and symbiotic agents. For each study, I am looking for...
28 July 2020 8,509 3 View
I wanted to design single precision floating point multiper using verilog plz provide me a source code for the same
11 April 2020 7,165 2 View
Lot of parametres impact the post listing behaviour of securities. How these parametres, collectively affect the behaviour? After IPO being floored, how can we assess these parametres with...
19 February 2020 1,764 3 View
Hello everyone, I want to perform electrostatic pull-in instability simulations of beams with various fixity conditions in ANSYS to get pull-in parameters. I want to compare these results from...
10 January 2020 5,075 0 View
Hello, I am interested in bolted joint for plastic & FRP composites. Can you recommend me any research or book related to this? Do you strictly include metal inserts & compression...
02 January 2020 2,613 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...
30 July 2024 6,794 0 View