Hello
We are currently producing several serotypes of AAV in our lab for molecular and preclinical studies using the cesium chloride density gradient. In production if AAVs, there is always a concern about the empty viral capsids and in various protocols purification methods is trying to minimize their presence. I have a question about their detection and normal range of their existence.
How can I detect them and quantify them? Is electron microscopy, the only way to detect them, or we have some other more convenient ways?
Besides, I hear somewhere that their prescence could be beneficial in prclinical in-vivo studies as they could attract a part of probable blocking anti-AAV antibodies. On the other hand, the excessive existence of them could interact with the effective transduction of target cells with the complete viruses. So, if I am true about above statements, could we define a reasonable range for amount of empty viral particles in AAV production?
Thank you in advance
Dear Vahid,
It is an interesting question. I would guess that the " reasonable range for amount of empty viral particles in AAV production" will depend on its intended application.
Anion-Exchange chromatography seems to be another method to distinguish between empty and full AAV capsids. Pls read more at : https://www.waters.com/webassets/cms/library/docs/720006825en.pdf
You might find some helpful papers/reviews in the Reference section of this webpage.
Good luck !
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