Hello, Last week I asked my PCR results and I send Sanger seq. Well, there is one colony including mutation, I guess. How to cleaning my sample for Sanger?
If you mean that your sequence is good up to a point and then messy then you may have an indel. In this case also sequence from the other end and manually read the 2 forward sequences and subtract the correct sequence from your dual sequence then align the sequence that is left to see the extent of the indel. Repeat in the reverse direction. You could also clone the sequence and then sequence clones to get a clean sequence.
Any software that reads minor peaks fom a .abi file will also indicate what the sequence is
Also, if you have other colonies that gave you the expected results, call the procedure a success and continue with your investigation. No need to chase down exactly what happened.