I have pancreatic sections that are H&E stained and I wish to measure their islet area to see the effect of the treatment given to the rats- indicative of insulin resistance levels. I have come across the software Image J that seems to be pretty good for the purpose but I need to adjust the scale to convert pixels into micro-meter in order to measure the area of islets. If you have any idea regarding the same, kindly help.
Hi,
If you have an image with a calibration scale (i.e. a ruler or a stage micrometer) open the image with the Image J, draw a straight line that defines a known distance on your calibration image. Now, in the “Analyze” menu select “Set Scale”. The distance in pixels will be filled in for you based on the length of the line you drew, and now fill in the 'Known distance' (according to your image with the ruler/stage micrometer).
Hope this helps!