What should be the minimum concentration for sequencing of a PCR product ?

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Aly Savadogo Popular answer

Dear Chowra

You can read this link

http://www.base-asia.com/dna-sequencing-services/sample-preparation

Samy Selim

50-100ng/ul of DNA but this depends on the company used.

Aly Savadogo

I agree with Aly Savadogo

Uma Kanta Chowra

Thank very much for valuable suggestions. Now I have a clear view about it.

Kevin Cavallin

We recommend .0025 µg/100 bases/µl, but we accept as low as 0.0005/100 bases/µl