For 1Kb PCR product
Dear Chowra
You can read this link
http://www.base-asia.com/dna-sequencing-services/sample-preparation
50-100ng/ul of DNA but this depends on the company used.
I agree with Aly Savadogo
Thank very much for valuable suggestions. Now I have a clear view about it.
We recommend .0025 µg/100 bases/µl, but we accept as low as 0.0005/100 bases/µl
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