Hi,

What doses of anti-IL-10 and anti-TGF-beta neutralizing antibodies I should administer in BALB/c mice for blocking IL-10 and TGF-beta cytokines respectively? How many doses should be administered and in what time span? I want to administer anti-IL-10 and anti-TGF-beta in separate groups of mice as well as both the antibodies in another (3rd) group simultaneously, then what should be the doses?

I want to study their role in infection, so whether I will administer these antibodies before infection or after infecting the mice?

Thanks