I have been trying to extract anthocyanin using different concentrations of alcohol. For that TPC, TAC and Antioxidant activity (DPPH method) are the following analysis that I'm performing.
So, the different method of extractions (different concentrations) shows different results.
For e.g.,
Suppose, Method 1 has indicated more antho content but the antioxidant activity is less.
Method 2 shows less antho or may be less TPC but more antioxidant activity.
So my question is
What should be the criteria for the selection of a method as i want both more TAC value and antioxidant content?
I'm highly confused , please help.
Your observations are right.
Results strongly depend on the experimental protocol.
I found a recent review on the matter (attached).
Hope it will help.
Check https://www.mdpi.com/2227-9717/8/11/1447/htm
https://www.mdpi.com/2076-3921/11/2/286/pdf
Also check https://www.researchgate.net/figure/Summary-of-the-most-common-extraction-methods-used-for-anthocyanin-extraction-for_tbl3_283492293
https://www.sciencedirect.com/science/article/pii/S0102695X18307336
Dear Mandeep Gulati . See kindly the following useful RG link: Article Up-To-Date Analysis of the Extraction Methods for Anthocyani...
Kindly see also the following useful RG link: Article Effects of Extraction Conditions on Bioactive Anthocyanin Co...
Also check please the following useful RG link: Article Effects of different extraction methods on antioxidant prope...
The following link is also very useful:
Article Alternative Extraction and Downstream Purification Processes...
Kindly check this paper
Article Up-To-Date Analysis of the Extraction Methods for Anthocyani...
Please follow the link.
Chapter Current Protocols in Food Analytical Chemistry
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