I am trying to measure intracellular GSH in Enterococcus faecalis. For this experiment I have to lyse bacterial cells. I am using sonication method. Last time I used 0.1 M PBS for lysis but I think I could not achieve. I just wonder that;

1) What should be the content of the lysis buffer for intracellular GSH measurement for sonication method?

2) How can we know if bacterial cells are lysed or not?

Thanks.