Can anyone help me to separate my phenolic compound in TLC with any new solvent system ?

My Compound is Leaf powdered Methanolic extract which is showing good phenolic compounds upon dipping in FeCl3. -My problem is phenolic compounds are not eluting with any common solvent system.

22 January 2021 4,693 7 View

Which journal is suitable to send manuscript related to survey on ethnobotanical usage of medicinal plants with no publication charges?

survey is related to diversity of medicinal plants used for treating different diseases

13 October 2019 9,985 2 View

No title

It seems that parenting style is considered as a constant pattern of parental behavior adopted by parents while dealing with their children. Can same parents adopt different parenting styles to...

11 February 2019 5,048 3 View

Can anyone help me out in interpretation of a powder XRD diffractogram?

I did the xrd for bentonite. I compared the peaks with the standard database but the peaks didn't match with montmorillonite. How is the analysis done?

04 August 2017 8,513 7 View

Difference in heat transfer properties of natural and synthetic graphite

explain the Difference in heat transfer properties of natural and synthetic graphite

22 June 2017 4,647 1 View

How I can coat porous metal, oxides nanowires or nanoparticles in the channels of 0.3 micrometer width and 0.5 micrometer depth????

suggest method of coating...

03 May 2017 4,382 1 View

I want to know how to search for promoter of any genes? Specifically I want the full promoter sequence of Aspergillus Nidulans alcA promoter?

I am planning to make an overexpression plasmid with alcA promoter and trpC terminater. Can anyone provide me the sequence of alcA promoter or even a plasmid map of such expression vector that you...

13 February 2017 857 3 View

How do you get rid of an oil globule-like substance forming during organic solvent extraction?

10 March 2015 1,392 4 View

What is the ideal temperature for organic solvent extraction as per the petroleum ether is concerned?

I am about to do organic solvent extraction but I am confused which one to use. The one with a temperature range of 40-60, 60-80 or 80-100.

10 April 2014 7,464 8 View

What is the bioactivity guided fractionation of natural products and does it apply to bacterial products?

Hi guys, I am planing to work on a marine bioactive compound. I came across a bioactivity guided protocol. Now I am confused about the fraction to be used for bioactivity. Which fraction...

18 March 2014 423 2 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

No title

01 March 2021 3,905 5 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View