That all depends on the abundance of your protein in the cell, the antibody you are using and the affinity of the interaction. Unfortunately there is no easy in IP. It's a lot of grunt work. I usually start just trying to get the antibody target out and working it out from there. 200 ug of protein is a good starting point. As for antibody use the amount suggested by the manufacturer to start.

Thanks Sarah. It will help me next time while doing IP.

Wael Thank you. I realized that i was not using enough antibody to pull down the protein. Thanks for your advice.