Deblina Guha

4 Questions 7 Answers 0 Followers

Questions related from Deblina Guha

What could be the reason for not getting the band of purified recombinant His-tagged p300 HAT domain protein at the desired position in SDS PAGE?

I have been trying to purify recombinant p300 Histone acetyltransferase domain (expressed in E. coli BL21 strain )using Ni-NTA His Bind Resin. At first I used 37 degree C temp 3 hrs for IPTG...

09 July 2025 5,181 0 View

Help for calculating cytosol to surface ratio of a protein using Image J?

I want to compare the above parameter for two samples. In one, cells are adherent and with full morphology on coverslip. While the others are non-adherent and round in shape. What I do is, using...

19 June 2016 7,277 1 View

What should be the amount of protein and antibody used while immunoprecipitating protein from cytosol or membrane fraction?

I want to study protein-protein interaction between integrin beta1 and EGFR in cell membrane and cytosolic fraction of HCT116 colon cancer cell line.

02 May 2016 6,156 3 View

Does 0.1% SDS in buffer disrupt protein-protein interaction?

While doing cell fractionation the nuclear lysis buffer contained 0.1%SDS. Following this I used the samples for immunoprecipitation where I did not get any positive result. My fractions were...

21 April 2016 1,374 7 View