In the intracellular MIC determination what should be the concentration of the amikacin and gentamicin for extracllular killing of mycobaterium tuberculosis, also what should be the treatment time, to avoid the penetration inside the macrophage.
For the similar type of study, but Salmonella typhimurium as the infectant, I used 80 uMolar Gentamicin for 30 minutes (I titrated this concentration from 15 minutes to 2 hours). It worked well for me.
Best
Multi drug resistant or(MDR) or extensively drug resistant(XDR) mycobacterial strains resistant to INH,RMP,can be treated with at least three injectable second line drugs i.e.,amikacin,capreomycin or kanamycin-not gentamicin.
Dear Sunil
200 ug/ml MBC and 20 ug/ml MIC is reported for amikacin to kill extracellular bacilli. generally people use 200 ug/ml for 1-2 hr then gives 2-3 washes with prewarmed PBS/RPMI.
Hope this helps
Good luck