I am supposed to sonicate 1 mg of carbon nanoparticles in 0.1 mL of MiliQ water using output control 3~27 W, 20 KHz for 5 min. I am also supposed to sonicate three samples of 130 microliters and one sample of 110 microliters of Borate Buffer with MiliQ water and the carbon nanoparticles at the same setting. What processing setting and and times are necessary on the Covaris E210 and M220?
Good time)
At first, 27W is too less for observed a sonofragmentation, especially for carbon.
For the second, what did you suppose a mechanism of fragmentation? Cavitation or Microjets?
I don't think that nanosize carbon can be a plane for a formation of the microjets. I suppose that, maybe, you'll observe sonochemical reaction, like
C+HOH=CO+H2 or something like this.
For the other hand, for the sonicate you must realize a high-pressure gradient, for example, what is difficult for nanosize object.
Hi,
Thanks for your response. According to a previous study by Aart van Amerongen, this sonication worked on Branson Sonifier 250.
http://cdn.intechopen.com/pdfs-wm/36216.pdf
"Neutravidin was conjugated to colloidal nanoparticles as described in several patents by van Doorn et al. (van Doorn et al. 1987, 1996, 1997). Briefly, a colloidal carbon suspension (Spezial Schwartz 4, Evonik Degussa Industries AG, Essen Germany) was prepared as a stock at 1% (w/v) in demineralised water. The suspension was sonicated for 5-10 min on ice using a Branson model 250 sonifier (output control 3~27W, 20KHz). This carbon suspension was diluted five times with 5 mM borate buffer pH 8.8 to give a carbon concentration of 0.2% (w/v), and sonicated for a second time as above. Neutravidin dissolved in 5 mM borate buffer pH 8.8 was added to the diluted colloidal carbon suspension at a concentration of 350 μg of protein per mL of suspension. The pH was readjusted to pH 8.8 and the mixture was incubated overnight by end-over-end mixing at 4 °C. Neutravidin-carbon conjugate was washed two times in a 5 mM borate buffer, pH 8.8, containing 1% (w/v) BSA, and re-suspended and stored in a 100 mM borate buffer, pH 8.8, containing 1% (w/v) BSA, 0.02% (w/v) NaN3 as a 0.2% (w/v) carbon suspension."
- Badges
- Science topic
- Similar topics
- Engineering
- Chemical Engineering