What role can 'in silico' methods play in experimental design?

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Can diamond be grown using molecular beam epitaxy?

22 July 2024 9,755 2 View

How can I put small FWHM in CasaXPS?

I'm trying to fitting Cu Auger peak into various state (Cu0, Cu+, CuI etc..) In Cu0, one of the peak's FWHM is 0.84 but when I try to enter smaller value than 0.89, "input error" is occured. How...

02 July 2024 4,932 3 View

Why PLLA deposited by gap electrospinning lose piezoelectricity?

I tried to prepare aligned PLLA nanofibers by gap electrospinning method. But after the deposition, when I tried to measure its piezoelectric sensitivity, it actually did not exhibit any...

17 June 2024 9,763 0 View

What are the causes of abnormal fluorescence PCR baseline?

Recently, I have been attempting to use fluorescence PCR to detect UGT1A1*28. I utilized the primers and probe reported in the paper "Rapid Allelic Discrimination by TaqMan PCR for the Detection...

22 May 2024 6,343 3 View

Plasmid DNA transient transfection reproducibility using liposome ?

Hi, I'm trying to find a pDNA transient transfection carrier for my MDA-MB-231. I'm using either liposome or lipopolyplex, but the transfection variability between experiments are too great. I can...

28 April 2024 1,189 2 View

Genotype binary -1/0/1 convert?

I have hapmap or Plink format. I want genotype binary -1/0/1 convert I want to create an input file in BGLR or BWGS to use gBLUP. If you have an R package or any other good method, I'd love to...

08 April 2024 8,268 2 View

Any recommendation for Shine Dalgarno or RBS or TSS Prediction Software?

Dear all, I am currently working on Acinetobacter baumannii and I wanted to look for the RBS or Shine Dalgano sequence of a gene for cloning. I have tried searching prediction online tools like...

01 April 2024 9,750 0 View

Alkali Blue 6B and Acid Blue 119 are same?

Alkali Blue 6B and Acid Blue 119 are the same compound in the measurement of Acidity in edible oil and fat? If not, color range by pH for those compounds is different? e.g. pH 9.4 (blue-violet)...

04 February 2024 5,692 0 View

What are some interesting topics at the intersection of NLP and neurolinguistics that would be suitable for undergraduate research?

As a starting topic for undergraduate research

16 October 2023 8,779 6 View

In latent profile analysis, how to draw a boxplot using standardized means?

hi i would like to draw a boxplot for the latent profile in LPA instead of curve lines and thx for the advice

30 September 2023 6,402 0 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...

21 July 2024 7,284 4 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

Neural Connectivity and Information Transfer?

Information transfer by the brain is dependent on the connectivity between neurons as mediated by learning (Hebb 1949). This connectivity is limited by the biophysical properties of neurons...

16 July 2024 4,249 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

How can we identify (in silico) the interacting amino acid residues or the nucleotides involved in the Protein-Protein / Protein-RNA interaction?

Hello! everyone, I am trying to study in silico Protein-Protein and Protein-RNA interactions. Now, is there any tool with which I can identify the interacting amino acid residues or the...

14 July 2024 950 2 View

How can i generate a CRISPR knockin mutation zebrafish model with a reporter?

Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...

14 July 2024 6,240 0 View