How to regenerate PES Membrane of centrifugal filtration tubes having 5 and 50kDa MWCO?

Dear Collegues, I am using a relevant number of centrifugal filtration tubes made of polyethersulfone (PES) with 2 different MWCO, 5 and 50kDa for separation of protein in milk. They are quite...

15 December 2020 7,951 3 View

How to sterilise maize seeds in the lab?

What is the most effective protocol to sterilise maize seeds in the lab? We have tried few common procedures but with no luck (still get fungus contamination on agar plates): 1)10% sodium...

19 November 2020 602 15 View

How I can simulate solid state sintering?

I want to simulate solid state sintering of metal powder, conducted under vacuum. I am interested in the initial stage of sintering, does not matter to reach full density. Do you know any model or...

24 September 2020 7,383 5 View

Is there a good library to perform Petri net simulation in python?

Hello, I have designed several petri nets for my research. I am wondering how can I simulate them and gather system performance measure (eg sojourn times, throughput) using python? Is there a...

11 May 2020 2,120 2 View

What are the relevant economic, technical and environmental aspects of the recycling of batteries for automotive applicantions?

A paper review. Could you please provide some researches about the topic?

28 April 2020 946 5 View

Difference between photovoltaic plant short-circuit current and synchronus generator short-circuit current in AC transmission system?

Hello, I understand that photovoltaic plants are able to disconnect very quickly if there is a fault in the grid (cause power electronics), while synchronus generators cant disconnect due to his...

30 October 2019 8,457 5 View

Psychic salience has been studied in his correlation with psychosis.What about psychotropic drugs effect?

Some drugs seems to raise the feeling of psychic salience while others dont.

20 September 2018 3,556 1 View

In psychotic patients, psychotic features of the speech can vary their gravity during an interview. What do you think can be the influential factors?

In my view, these factors can be: the emotional meaning of the theme for the patient, anxious sympyoms caused by the situation or the interviewer, a relatedness feeling with the interviewer, a...

03 September 2018 303 3 View

Which assay to detect human serum levels of anti-troponin-I, anti-troponin-T and anti-tropomyosin antibodies?

We are currently using indirect ELISA to measure human serum levels of anti-troponin-I, anti-troponin-T and anti-tropomyosin antibodies? This indirect ELISA is not sensitive enough for our...

23 May 2018 6,546 2 View

Ksp in aprotic polar solvent , any idea?

11 May 2018 2,772 2 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Can anyone identify this fungus/mushroom from China?

The pictures were taken in Guishan Mts in Yunnan. The fungus is ca. 10-12 cm high.

07 January 2021 6,639 10 View

Can forward and reverse sequences of PCR products be entirely different from each other?

Dear All, The forward and reverse sequences obtained from the PCR products are not identical at all. Can I still make the contigs for doing BLASTn? Individually both sequences are showing the...

22 December 2020 5,280 5 View

In this sterile needle biopsy wet-mount, does the motility pattern fit with Oomycete zoospores?

We are examining tissue from a cluster of human and canine patients with a similar pattern of systemic illness of unknown cause. All members of the cluster have evidence of motile zoospore-like...

23 October 2020 4,480 3 View

Complete protocol for Fungal Genomic DNA isolation?

Dear all, I am looking for recommendations for an efficient protocol for the extraction of Genomic DNA from several fungal cultures. I have most of the chemicals and equipment used in the process,...

20 September 2020 7,906 7 View

Is there a valid alternative way to replace the use of camera lucida (or drawing tube) for scientific and taxonomic drawings?

So far I have not been able to find a camera lucida that fits a low range microscope.

05 September 2020 3,342 4 View

Where can I download the software Quant for leaf disease assesments?

10 June 2020 7,067 3 View

These images may show a novel/emerging human pathogen- Do you think it is consistent w/ oomycetes/myxomycetes/fungus? Anyone pursuing this already?

I am a member of a group of veterinarians who all became ill within about a years time of each other, and who all were employed in the same physical location. Some of the affected veterinarians...

02 June 2020 2,248 19 View

How to isolate/quantify a fungal enzyme for comparison of control vs. treatment?

I want to compare the amount of an enzyme's expression (laccase for example) or presence in the tissue as a result of the experimental treatment. Is there a protein similar to an antibody that...

31 December 2019 1,359 2 View

What are the benefits of depositing a fungal culture in noted fungal culture collections?

During my service as Mycologist in Department of Medical Mycology, in Calcutta School of Tropical Medicine, we isolated many interesting fungi. But only a few of them are deposited in CMI, ATCC...

31 December 2019 8,522 4 View