If we want to relate changes in NADH/NAD ratio to increase/decrease in production of certain metabolite in a bacteria, then what range of ratio changes will be considered significant?
Low ratio may be due to aerobic condition and high may be attributed to anaerobic. The presence of e acceptor play an important role in deciding this ratio. This article may help you. http://jb.asm.org/content/181/8/2351.full.
the ratio depends on the metabolite required
for butanol production check this paper for detail
Meyer CL, Papoutsakis ET (1989) Increased levels of ATP and
NADH are associated with increased solvent production in
continuous cultures of Clostridium acetobutylicum. Appl Micro-
biol Biotechnol 30(5):450 – 459
using exogenous electron shuttles such as ferrihydrite, anthraquinone disulfonate, methyl viologen (MV) or change in oxidation reduction potention by using Na2S, cystiene might help
thnku so much for the suggestions.. my question is that of i want to say that NADH/NAD ratio affects production of say ethanol in an organism in two different conditions say with two different substrates, then what range of change in NADH/NAD ratio will be considered significant means can i say if my ratio is changing from say 0.4 to 0.8 then it might be affecting the production...?? i hope i am clear with what i have in my mind...
For a specific condition, the redox level might not be same as with other condition. It applies same for the use of different substrate. However, the ratio is directly related with redox and it will effect the product formation. Therefore, doing a back calculation, I think you may get some idea of electron flow. that will give you an answer.
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