Chandan kumar Gautam

10 Questions 25 Answers 0 Followers

Questions related from Chandan kumar Gautam

Could you please help in troubleshooting a protocol for 'total inorganic phosphate quantification from plant samples, or suggest a different protocol?

Hi there! I am trying to quantify total inorganic phosphate from plant samples using a spectroscopy based techniques. I am facing issue with the standards. I have not tested the samples yet. For...

22 July 2022 7,198 3 View

How can I avoid any possible chances of getting algae in my rice hydroponics system?

I use light protected jars and big falcon tubes to grow my plants. Usually, I don't get algal contamination when growing plants for 2-3 weeks. But, sometimes I get algal growth in my samples. It...

24 June 2019 2,160 4 View

My T-DNA line, miRNA lines and over-expression lines showing similar phenotype under stress?

I am working on characterizing the function of a gene under stress. The T-DNA lines were obtained from SALK. The miRNA was prepared in lab and it shows very insignificant expression of my gene....

12 February 2017 9,699 3 View

How can I prepare PEG infused 1/2 MS media?

I want to prepare 1/2 MS supplemented with PEG, in % (1.5, 3.0, 4.5 and 6.0). I read in an article (J. Exp. Bot. (2000) 51 (350): 1555-1562) that equal volume (i.e., PEG volume= volume of 1/2 MS...

03 October 2016 6,077 4 View

What is the time required for INS 1 cell to adhere and multiply ?

What is the time required for INS 1 cells to get adhere and multiply themselves? What may be the duration required for INS1 cells to secrete insulin? And, what is the shelf life of the INS1 cells...

05 March 2015 1,158 3 View

Are INS 1 cell lines suitable for insulin release studies?

Currently, I am working on RIN5 cell lines for studying stimulated insulin release. These cells are very slow growing and take a relatively longer time for attachment. So, I am thinking to...

27 February 2015 2,948 4 View

What is the shelf life of lab prepared Krebs-Ringer Bicarbonate Buffer?

I have prepared KRB buffer in lab for animal cell culture experiment. Can anyone let me know the shelf life of this KRB solution. I have stored this buffer at 4 degree celcius.

05 February 2015 2,477 2 View

What are the solvents used to dissolve the standard (Diazoxide, Glibenclamide, Sulphanylurea) drugs for insulin assay of Rat Pancreatic beta cells?

What are the solvents used to dissolve the standard (Diazoxide, Glibenclamide, Sulphanylurea) drugs for insulin assay of Pancreatic beta cells of rat growing in RPMI media? I am performing a...

20 January 2015 7,072 4 View

At which RCF/RPM and temperature should a cooling centrifuge be operated to separate bacteria from nutrient broth ?

I am working with the microbial metabolites. I need to separate the bacteria (Enterobacter) from the nutrient broth (LB) without rupturing its cell wall. Further, I need to lyse the bacteria in...

22 January 2014 9,436 8 View

What's the simplest method to isolate omega-3 long-chain polyunsaturated fatty acids from plant parts?

I am working on isolation of omega 3 fatty acid from flowers. Please suggest the simplest way to isolate it.

11 January 2014 6,403 2 View