Color at the test line of a lateral flow imuunoassay strip ?

Hi, I am trying to develop a competitive lateral flow assay to detect a protein. I coat the protein on the test line and use gold nanoparticle-antibody as the conjugate that binds to the test...

14 February 2019 2,153 4 View

What is the characteristic UV-Vis Spectrum of Ultra small Gold Nanoparticles ?

Hi, I am trying to synthesize ultra small 2nm gold nanoparticles by NaBH4 reduction (Brust Method). What should be the characteristic UV-Vis spectrum of 2nm size gold nanoparticles. Should we or...

18 September 2018 4,460 9 View

What are the ways to inactivate a Virus without affecting its ability to bind to its antibody ?

We are trying to detect a Virus using a sandwich lateral flow assay. We are targeting the epitopes on the envelop protein of the virus. Is there anyway I can inactivate the disinfect the virus,...

15 March 2018 2,631 3 View

How much more difficult it is to detect a whole virus when compared to its Non Structural (NS) Protein ?

I am trying to develop a gold nanoparticle based lateral flow sensor to detect a whole intact Virus. I have tried with 3 different antibodies of the virus. But, I was not successful. Reading the...

08 February 2018 3,611 8 View

Is there any ideal initiator for synthesis of PMMA Nanoparticles through emulsion radical polymerisation ?

I would like to know whether ammonium per sulfate (APS) is a good radical initiator for synthesizing PMMA nanoparticles by emulsion radical polymerisation. Or, does it have any drawbacks...

04 October 2016 7,812 8 View

Can anyone help me with designing a portable sensor using a nitrocellulose membrane ?

I need to develop a portable low cost sensor using a nitrocellulose membrane (NM). I have done an assay for my analyte on a 96 well plate using monoclonal and polyclonol antibodies and a labeling...

31 May 2016 9,650 1 View

Any advice for absorption spectrum coming in the negative region of the y-axis in a UV-vis spectrophotometer?

In a UV-vis apectrophotometer, what does it mean if the absorption spectrum is coming in the negative region of the y-axis (absorbance) even after taking a baseline with reference?

25 December 2013 3,859 21 View

Can anyone help me with design of paper strip for lateral flow immunoassay ?

I have to develop a paper strip for detection of a small molecule, a nucleoside. I need some suggestions on the arrangement of different pads as I am doing the paper based sensor for the first...

01 January 1970 4,405 7 View

Is Lateral Flow Assay specific to clonality of the anitbody ?

01 January 1970 7,976 12 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View