The procedure compute?

02 March 2021 5,204 3 View

One-sided testing of MLM in R?

Dear colleagues, Does anyone know how to conduct a one-sided testing of regression weights (MLM) in R? The actual model is fm1rs

23 January 2021 3,970 3 View

[Measurement invariance] - Do you test it on the full SEM model, on each construct separately, or both?

Hi everyone, I would like to know whether both cultural groups interpreted my questionnaire in a similar way. To do so, I conducted a measurement invariance analysis on the whole model of latent...

14 January 2021 1,845 3 View

Is it possible to do a closed loop control of a sine wave?

An analogue voltage sine wave signal is send from a PLC to a Piezo actuator. This actuator converts the voltage to force. A load cell checks what the applied force is and sends a mA-signal...

27 December 2020 7,037 3 View

[SEM] Why RStudio and AMOS report different p-values (but same estimates)?

Hi everyone, I have conducted a SEM analysis in AMOS then replicated it in RStudio. While I obtained identical estimates and model fit statistics, the p-values differ between software programs...

25 December 2020 3,669 4 View

What should my boundary conditions be in abaqus and how do I apply a sine load?

I want to model a T bone specimen that is subjected to a sine load. The specimen is clamped in as showed on the figure. I was wondering what I should chose as boundary conditions and how I can...

08 November 2020 9,766 4 View

Does anyone know instruments to measure "mental health literacy" in teachers?

Hi there, I am looking for questionnaires measuring mental health literacy in teachers and educators. does anyone know any studies in this area?

16 September 2020 1,682 4 View

Is there any possibility to get the estimated values (listed for every participant) of a specific latent variable in R or Mplus?

Is there any possibility to get the estimated values (listed for every participant) of a specific latent variable in R or Mplus?

17 June 2020 3,819 3 View

Literature for multilevel MCMC models?

Hey there, I am looking for literature or tutorial videos, which are concerned with the question of how multilevel markov chain monte carlo models are conducted in R or Mplus. Can someone help...

20 April 2020 7,801 1 View

How to generate a ROC-Curve for multilevel regression models?

Dear colleagues, Does anyone know, how to build a ROC-Curve and a AUC-Value concerning multilevel logistic regression models using R or Mplus. I would be very grateful for any articles,...

26 February 2020 8,930 3 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there a way to grow biofilm in lab to mimic biofilms in showers and baths? Also, how can I measure it?

Hello, I am new to biofilm research and have a question about growing and measuring biofilms. We want to research the effect of a type of enzymes in breaking down biofilms in showers, baths, hot...

14 February 2021 3,522 3 View

What is the method of removing RNase contamination from a filter paper surface?

I am working on a biosensing project on paper substrate, however, it is found that the RNase contamination occurs on the filter paper. Might I ask is there any method to clean the paper surface...

12 February 2021 3,926 3 View

How do I stop losing peak shape with Waters T3 Atlantis Column?

I am using this column with a gradient of acetonitrile vs a phosphate buffer. I clean the column after every run with 2%ACN:98%H20 and store in 30% ACN. I've noticed that my peaks are getting...

10 February 2021 9,181 1 View

How to prepare OH- terminated Si Wafer?

Hi, Folks. I am working on the ALD project to deposit Al2O3 on the Si wafer from TMA and Water. I have struggled with the preparation of OH- terminated Si wafer. I have both Si(1 0 0) and...

08 February 2021 8,792 2 View

How to clean up an already pure protein from grwoth medium?

Hi all, I have got a protein, expressed in P. pastoris vector, more than 97% (SDS-page) pure in the culture medium after cells separation. Despite the protein expressed has a his-tag, Nichel...

04 February 2021 5,173 6 View

Can evaporation of PCR reactions lead to contamination in the cycler?

The whole volumes of my PCR reactions (10 uL) evaporated during the cycling (honest mistake, I know why it happened and how to fix it - wrong PCR caps used). My question is, could this leave some...

23 January 2021 3,887 4 View

Can anyone suggest an RNA extraction protocol from grape berries?

I am trying to extract RNA, from grape berries of a red variety, for qPCR (to study gene expression). I have tried nucleozol and a CTAB protocol (with b-mercaptoethanol+boric acid + cleaning with...

18 January 2021 8,857 2 View

I need to do a qRT PCR but there are not available filter tips?

Due to the COVID situation it is nearly impossible to find filter tips. Is it possible to do it without filter tips by making sure to clean the pipette with RNAse zap before use and working...

17 January 2021 8,839 3 View