What Primer should be use on gastrointestinal bacteria of catfish to identify the microbes for DNA analysis?

01 January 1970 1 1K Report

Hello, I need to run PCR on the DNA I found on several unknown microbes I collected from catfish. Which Primer should I use and where can I fin it? As well as what is the best method to do this.

Thank you in advance

Badges
Science topic

More Veronica Alston's questions See All

Mixed ANOVA - help?

I'm currently working on a piece which asks to conduct an ANOVA - looking at the effect of sporting group (2 levels), and exercise trials (two trials with six levels). I've computed this all...

27 February 2021 9,626 4 View

Which is the best mitochondrial potential dye for primary cells?

Hi! I am wondering if someone can recommend me a good dye for measuring mitochondrial potential. I have used TMRE with good results in the past, but I have heard also about Mitoview. I would use...

12 January 2021 4,200 4 View

How to analyze sodium lauryl sulfate using HPLC-RID?

Hi, I am analyzing the content of anionic surfactant: sodium lauryl sulfate (SLS) in commercial detergents using HPLC-RID/refractive index detector. I used column Agilent Zorbax Eclipse XDB C-18...

15 November 2020 1,405 2 View

How do I test for gender differences in Spearman rho correlations?

I want to explore if there is a gender difference in a correlation between two ordinal items. I have calculated spearman rho within gender for the items, but I now need to test if the correlation...

09 October 2020 970 13 View

How to get rid of Matrigel during enteroid passaging?

Hello. I am trying to generate monolayers from cow enteroids. The protocol I am following involves 0.5uM EDTA and Trypsin-EDTA 0.05%, each enzyme is applied in two different steps. But for some...

10 March 2020 1,805 1 View

Is there a way to edit the citations in MENDELEY so it's in the order of citation in the text body instead of Author?

Does anyone know how to change the citation style in Mendely in order to sort the references according to the order of citation in the paper? So that in the text is displayed only the number (eg....

01 January 2020 746 2 View

How do you feel about the current state of the job market for Pharmacists in the United States?

As 2020 comes to a close are you optimistic or pessimistic about the future of Pharmacy Practice in the USA? If you are from another country please answer but Identify your home country

20 December 2019 7,486 2 View

How to avoid impurities in immunoprecipitation with magnetic beads?

Hello, I need to extract an antigen from human serum. I tried with streptavidine-coated magnetic beads and a pair of biotinylated antibodies. Then I followed the elution procedure, as recommended...

09 September 2019 6,947 4 View

How to analyze alkyl distribution of Methyl Ester Sulfonate / Sulfonated Methyl Ester?

I need to analyze the alkyl distribution of Methyl Ester Sulfonate to know the molecular weight of my sample. I tried to use gas chromatography to do this. My method was to reflux the sample first...

14 July 2019 2,133 4 View

Can you recommend me some articles that dealing with the electronic transitions of toluene?

Hello! I'm working on assigning absorption bands to UV-vis spectra of unknown matrix. I think some are due to the presence of toluene, but I can't find any article that describes the electronic...

19 March 2019 8,119 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View