Hi Meredith,

I used to perform fluorescent western blot in an Odyssey InfraRed Imaging System from Li-Cor biosciences with fairly good results (First link below). Which system are you using? Is it an IR or a non-IR system? This is important for the choice of secondary Abs.

In the Odyssey system we used nitrocelulose membranes, but PVDF ones can be used as well. We purchased the blocking solution from Li-Cor biosciences as well as the IRD-labeled secondary Ab's (the fluorescent ones; see second link M&M section).

Here you can find different protocols and useful tips to perform Fluorescent western blots in this imaging system (https://www.rockland-inc.com/uploadedFiles/Support/Protocols/Odyssey-Western-Blot.pdf)

I'm sorry to tell yoy that I don't have any experience about alpha spectrin.

Hope it helps.

Best

http://www.biomedcentral.com/content/supplementary/1471-2202-14-127-s2.pdf

Article Efficient synaptic vesicle recycling after intense exocytosi...

Thanks for the resources! I am using the Odyssey Infrared Imaging System.