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09 October 2017 4,100 36 View

How often and in what situations must the grounds of widely distributed theories of natural processes and phenomena be revised?

07 August 2017 9,704 3 View

DNA origination and replication or protein formation, what is the quintessence of the life?

09 October 2014 961 8 View

Why is the volume density of living organisms over the world ocean column much less than that over the soil, near-surface, and near-seabed layers?

In nature, all phenomena, processes, and their features are inter conditioned, and any correct phenomenological theory of a principal natural phenomenon should explain an ensemble of phenomena...

02 March 2014 2,790 2 View

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This question is of principal importance when discussing the problem of the living-matter origination mechanism.

09 October 2013 5,457 16 View

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07 August 2013 335 15 View

Do you know of any proofs for the independence of the gravitation coefficient from the star age and size?

The fact is that, until the gravitation coefficient independence from the star age and size is proved by independent measurements, the notions on the so-called dark matter, dark energy, etc. are...

05 June 2013 3,791 4 View

Are solutions of the problems of dark matter, black holes, etc. hidden in celestial phenomena or in our heads, i.e., in erroneous notions on stars?

The so-called standard solar model and all calculations of the masses and rates of stars are based on arbitrary Eddington's (1919) notions on the stars as on the balls of an ideal gas, which...

05 June 2013 2,892 4 View

A funny question: bacteria in the brain; do they migrate thereto or originate therein?

In the recent years, bacteria are discovered in human brain. However, the moment and history of their appearance there is not clarified.

01 January 1970 3,342 24 View

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01 January 1970 9,227 11 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View