Hi,
I am searching for maturation rate of GFP. I did not find anything solid in literature. I notices that some researchers have mentioned that certain receptors tagged with GFP are about 70% fluorescent. They have claimed that due to misfolding or maturation they are 70% fluorescent. Any solid explanation from an expert will be appreciated.
Hello
kindly look at links here
Good Luck
https://greenfluorescentblog.wordpress.com/2012/08/01/folding-and-maturation-or-how-to-evolve-your-own-gfp-part-1/
http://pubs.acs.org/doi/abs/10.1021/jacs.7b00676
When you have certain receptors tagged (or fusion) with GFP, this extra part of 'receptor' structure can affect the 'folding' of GFP protein into its nature 3-D conformation. Therefore, when a receptor or other protein is needed to attach to GFP, researchers prefer to try fusing it to either N-terminus or C-terminus of GFP and find out which structure will turn out better (see attachment).
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