For better resolution of protein, what percentage will be better, and also for two different buffer ( for stacking and resolving buffer) only Tris-Cl of pH 6.8 and Tris-Cl of pH 8.8 is required or should I add SDS in to it to make it Tris-SDS ?
Man, its been awhile since I poured my own gels. But I do seem to remember that you should not use tris-HCl, but tris-glycine.
You don't need to have SDS in the acrylamide, just in the running buffer.
Their is no perfect gel concentration/composition for all proteins.
You can read this protocol which is very instructive to help to design your experiment.
gel
https://www.google.de/url?sa=t&rct=j&q=&esrc=s&source=web&cd=2&cad=rja&ved=0CEwQFjAB&url=http%3A%2F%2Fwww.bio-rad.com%2Fwebroot%2Fweb%2Fpdf%2Flsr%2Fliterature%2FBulletin_6040A.pdf&ei=tdvqULLuMMrSsgauzoGwAQ&usg=AFQjCNEF1D4YAbo9Q-eUn1NZFNVFo80-XA&sig2=CACHNUZYoPxx3y6c_6v0Ng&bvm=bv.1355534169,d.bGE
blotting
www.bio-rad.com/LifeScience/pdf/Bulletin_2895.pdf
Good reading
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