What PCR reaction are you using for the detection of the malaria parasite?

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What are the roles of innovation in achieving the Sustainable Development Goals (SDG)?

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Amal Mahmoud

Follow the kit protocol

Craig W Duffy

Most of the time we use a protocol based off of this paper: A genus- and species-specific nested polymerase chain reaction malaria detection assay for epidemiological studies. ( http://www.ncbi.nlm.nih.gov/pubmed/10348249 )

Sanjay Arvind Desai

Depends on the species. If you are interested in P. falciparum, you may want to use reduced annealing and extension temperatures because of the AT rich genome. We generally anneal at 47 oC and extend at 2-4 oC below the melting temperature of the primers.