How to evaluate motivation in scholars, teachers and students who work from home during pandemic?

Dear all, What tool can be used to measure motivation in scholars, teachers and students who are in home isolation or work remotely? Any specific tool suitable for all people involved in Academia...

08 December 2020 7,968 10 View

How to replace the liquid blocker?

Dear colleagues, In my immunofluorescence stainigs I use liquid blocker pen, before solution with 0.3% Triton X-100. This helps to avoid dripping of solutions with primary antibodies and Triton...

30 September 2020 9,134 4 View

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06 July 2020 4,133 2 View

How long can one store primers?

Dear colleagues, one year ago we put our liquid primers in a high concentration into a -80 fridge. Can we still use them for the qPCR?

11 May 2020 4,521 16 View

What is an acceptable small sample size to carry out principal component analysis for a rating scale with 16 items???

Hi Can someone tell me what would be an acceptable sample size for principal component analysis for a rating scale with 16 items. Thanks!

30 March 2020 9,756 3 View

How to insert image in introductory text of survey in Opinio?

Hi I need some technical help. I am trying to insert an image in the introduction of a survey in Opinio. I click on 'insert image' button, then I am prompted to paste the url of the desired image,...

28 March 2020 1,543 3 View

How does the Excel work with GRG algorithm?

I'm struggling with my qualification work. Excel methods solve the math problem easy, but I don't catch the idea how he does it. I need to understand it to create the code or just to describe the...

12 March 2020 6,567 6 View

What to do if Fatal error: Atomtype LC3 not found and force field is fine?

When I was simulating my protein, "Fatal error: Atomtype LC3 not found" had occured. I checked my force field atom type and it contains LC3 with correct parameters. Before this error I used...

10 March 2020 2,189 2 View

Has anyone optimized ViromerRED in THP-1, H9, and MCF7 cell lines?

I am currently attempting to optimize ViromerRED transfection in THP-1, H9, and MCF7 cells. Our initial transfections with the first batch was promising, but the new viromer we received doesn't...

15 October 2019 5,053 4 View

Can anybody explain why does the line on gel disappear after 15 cycles of PCR?

We make test PCR to choose optimal quantity of cycles and amount of cDNA for amplification, and there are lines on 9 cycles and 12 cycles (same sample), but the line on 15 cycles disappear (also...

02 April 2019 3,158 7 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View