I'm trying to characterise the pheromone of a moth composed of 10-14-carbon alcohols and acetates using GC-MS. I do not need to perform a full ion scan, I would only need to scan from m/z 39 - m/z 350 at most. I have set the MSD parameters to get about 4-5 scans/peak. Besides that, is there anything else I should change? Should I perform some sort of calibration?
Thank you in advance
If your GC-MS is already set up, you may not have to calibrate the MS. However, it won't hurt to recalibrate. You may want to check out
http://www.waters.com/waters/en_US/Quantitation-and-Calibration/nav.htm?cid=10091170
for more information. I would always run a commercial standard of you compounds of interest if available to obtain retention time and spectra based on your system and conditions. That would be the safest way of identifying your analytes. You may also choose to spike the standard into your sample for confirmation.
If you yet have to identify some or all of your analytes, you can also determine the Kovats index, which is basically the retention time relative to the retention times of alkanes analyzed under the same conditions. An alkane standard containing C8-C20 is readily available for purchase. You can then compare your Kovats (and more) to a database, such as
http://webbook.nist.gov/chemistry/
Good luck!
Yes Im agree with above answer and you first try to do calibration and set the mass.
good luck!
Thank you very much for your answers. We have already calibrated the equipment. I'm going to run some standards to check their spectra
Im agree with above answer and you first try to do calibration and set the mass.
- Badges
- Science topic
- Similar topics
- Invertebrate Zoology
- Entomology
- Insect
- Neoptera
- Holometabola
- Moths