Ricardo E Castro

8 Questions 41 Answers 0 Followers

Questions related from Ricardo E Castro

Which is better, NGS or a commercial blocking solution for immunohistochemistry?

We want to characterise some brain regions in an insect using an anti-synapsin protein. Most protocols use Normal Goat Serum to dilute the antibodies and as a blocking solution, but it seems to be...

07 July 2018 3,983 6 View

How to make ultra thin sections of antennal sensilla? Any tips?

We would like to characterise the antennal sensilla of a moth using TEM. Does anyone have any tips about ultra thin sectioning? I have read several protocols about it, but none of them describe...

02 February 2018 975 4 View

Could anyone send dead adult moths/trichopterans?

Hi. We are analysing the external morphology of adult moths, so we would like to check as many specimens as possible, mainly pests. If possible, we would also like to compare them with...

06 June 2016 6,321 10 View

Chemoreceptors not staining with crystal violet. What could be wrong?

I'm trying to stain the chemoreceptors of male and female moths using Slifer's crystal violet method. I immersed the whole moths (which are about 1.5 cm long) in an aqueous solution of 10%...

05 May 2016 9,192 7 View

How do insects manage to eat plants with a high content of calcium oxalate crystals?

I was reading an article about how raphides (needle-like calcium oxalate crystals), together with proteins from the plant, are used by some plants to deter herbivory. However, there are plenty of...

01 January 2016 2,691 5 View

What other parameters should be changed to acquire ions at a specific mass range in a GC/MS system?

I'm trying to characterise the pheromone of a moth composed of 10-14-carbon alcohols and acetates using GC-MS. I do not need to perform a full ion scan, I would only need to scan from m/z 39 - m/z...

05 May 2015 8,435 4 View

Must insects be alive when fixing them for Transmission Electron Microscopy?

We want to characterise the ultrastructure of some glands in Lepidoptera using TEM (we would like to observe the cell types, tubules, etc), but our insects are seasonal, with only one generation...

05 May 2015 10,120 12 View

Can someone help with mite identification?

I found some mites running on some Agave plants we have in our greenhouse (attached picture) and just got curious about their identity. They have about 17 spatulate setae around the edge of the...

04 April 2015 5,236 9 View