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Questions related from Ricardo E Castro
We want to characterise some brain regions in an insect using an anti-synapsin protein. Most protocols use Normal Goat Serum to dilute the antibodies and as a blocking solution, but it seems to be...
07 July 2018 3,964 6 View
We would like to characterise the antennal sensilla of a moth using TEM. Does anyone have any tips about ultra thin sectioning? I have read several protocols about it, but none of them describe...
02 February 2018 954 4 View
Hi. We are analysing the external morphology of adult moths, so we would like to check as many specimens as possible, mainly pests. If possible, we would also like to compare them with...
06 June 2016 6,300 10 View
I'm trying to stain the chemoreceptors of male and female moths using Slifer's crystal violet method. I immersed the whole moths (which are about 1.5 cm long) in an aqueous solution of 10%...
05 May 2016 9,173 7 View
I was reading an article about how raphides (needle-like calcium oxalate crystals), together with proteins from the plant, are used by some plants to deter herbivory. However, there are plenty of...
01 January 2016 2,676 5 View
I'm trying to characterise the pheromone of a moth composed of 10-14-carbon alcohols and acetates using GC-MS. I do not need to perform a full ion scan, I would only need to scan from m/z 39 - m/z...
05 May 2015 8,421 4 View
We want to characterise the ultrastructure of some glands in Lepidoptera using TEM (we would like to observe the cell types, tubules, etc), but our insects are seasonal, with only one generation...
05 May 2015 10,103 12 View
I found some mites running on some Agave plants we have in our greenhouse (attached picture) and just got curious about their identity. They have about 17 spatulate setae around the edge of the...
04 April 2015 5,216 9 View
