How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How can I force Gaussian to search the exactly point group of my molecule?

Hi, I want to know the point group of my molecule "adenine-thymine" , it is composed by 2 molecules ( thymine and adenine) and both of them belong to Cs point group. What can i do ?? help please.

02 December 2020 7,091 3 View

How is isolation of outer membrane vesicles of Neisseria gonorrhoeae done?

Procedure for isolation of Neisseria gonorrhoeae outer membrane vesicles as vaccine antigens.

01 October 2020 8,320 3 View

COVID 19 have multiple causes?

I think the s.c. COVID 19 pandemic clinical manifestations (syndromas) have many different infective causes - Corona viruses (enteroviruses with many subtypes), othe viruses and bacterias! It is...

24 May 2020 5,842 5 View

A vaccine effective in monkey against corona virus can be 100% effective in human?

An experimental COVID-19 vaccine protected monkeys from pneumonia and prompted a strong immune response in the animals. Vincent Munster at the National Institute of Allergy and Infectious...

15 May 2020 7,939 6 View

How a deep learning approach detect that the pneumonia is caused by Covid-19 from an x-ray?

24 March 2020 6,877 3 View

How AI and Deep Learning can help to Screening Corona virus (COVID-19) ???

There are many Articles out there related with Deep Learning with corona virus, For Example : "deep learning algorithm using CT images to screen for Corona Virus Disease (COVID-19), Deep Learning...

12 March 2020 3,780 9 View

Have I got the settings right?

I want anyone to be able to download my paper "elderberry is antibacterial, antiviral etc" for free and easily. Have I got the settings right? With Covid-19 spreading I think elderberry (Sambucus...

02 March 2020 6,607 3 View

If the cofactor(NAD) concentration in 1mM and substrate is 10mM in enzyme reaction, the substrate become 9mM after reaction?

If the cofactor(NAD) concentration in 1mM and substrate is 10mM in enzyme reaction, the substrate become 9mM after reaction?

02 March 2020 3,982 6 View

What are the necessary parameters for measuring GAPDH NADH production over time in a UV transparent 96-well plate?

I am looking to measure NADH production at 340 nm by GAPDH (from crude protein extract) over time in a UV transparent 96-well plate. So far I am unable to detect any signal and I was wondering if...

12 February 2020 7,376 2 View

Chloroquine as an effective prophylactic for 2019-nCoV in humans ?

A study in 2005 tested in an experimental setup the efficiency of chloroquine to stop the coranavirus S spike from developing and to ace 2 enzyme thereby stopping transmission and cutting the...

22 January 2020 5,956 7 View