22 February 2023 0 3K Report

I will use Illumina equipment for know diversity of extracellular bacteria of human tissue and urine samples by using Quiagen Kits.

What is the name of kits?

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Why citations are not recorded on my profile researchgate web?

Dear colleges , I would appreciate if you have a time to help. I received mails told me there are numbers for research articles. These citations are not recorded on my profile. Regards, Sherif

09 July 2024 6,195 1 View

How can I induce type 2 diabetes in C57 female mice?

Hi everyone, I tried many published protocols to induce type 2 diabetes in c57 female mice using high fat diet for 1 month then STZ 40 mg for 4 days or 50 mg for 5 days followed by 10% sucrose in...

18 April 2024 4,680 2 View

How can I create a citation/citation for a specific research found on the website?

كيف يمكن عمل استشهاد/ اقتباس لبحث معين موجود علي الموقع ؟

06 April 2024 9,898 1 View

Understanding PCA plot for MD simulations ?

In this figure, a pca plot is shown for different protein , protein complex systems depicting the essential subspace for these systems. I want to know how can I interpret these plots ? What...

31 March 2024 9,951 3 View

High dsDNA concentration on Qubit but horrible, degraded gel electrophoresis?

Hello everyone! I am working on my undergraduate thesis. After I used the DNeasy® PowerSoil® Pro Kit to extract DNA from a coral mucus sample I use 2 microliters of DNA to measure the output's...

15 December 2023 4,749 1 View

Is there any bioinformatics tool to predict nanobody sequence based on antigen sequence as an input ?

We all know that nanobody development is time and money consuming, it nearly needs a grant. I'm wondering if there is any bioinformatics tool or a method to predict nanobody sequence against...

09 January 2023 8,310 2 View

What is the meaning of the dynamic distribution of pesticides on plants?

What are the most popular models used? And how to apply it in practice to a crop? and, What are the best references to look at on this point? Thank you so much

28 June 2022 2,112 0 View

I want to simulate 3d transient thermal storage (Cubic block of concrete with a hollow cyl contains hot stream), what is the best Ansys solver used ?

Thermal Transient or Fluent! I have tried to use transient thermal but the results show that there is no Temperature Gradient in the flow direction. So how can I let the flow temperature change...

01 November 2021 3,819 3 View

How to use GFI model in abaqus? and when does it becomes necessary to use it?

hello, i am modelling a RC slab in Abaqus, the slab is supposed to fail in punching shear which is a sudden failure. i defined the nonlinear behaviour of concrete using concrete damage plasticity...

23 October 2021 9,555 3 View

In abaqus, is it ok if the only stress acting on the longitudinal steel bars in a RC flat slab is S11?

Hello, i am modelling a flat slab that is loaded upwards at its centre. the slab is reinforced with two meshes of steel bars ( upper and lower mesh), and it's supported at all it's edges. i found...

14 October 2021 5,054 4 View

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Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,081 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

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RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

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Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

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Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,101 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,056 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

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Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

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