How to amplify extremely high GC-rich template?

I am cloning a whole cDNA of a particular gene and I have designed forward primer at start of 1st exon. The region downstream of forward primer is extremely high GC rich (around 85-89%). Because...

17 September 2020 6,571 11 View

How to apply load in abaqus ?

I have created a beam and i dont know, what is the maximum load it can take, Is there any way to increase the load continuously without giving a fixed value, so that at some load the beam will...

02 September 2020 6,246 9 View

I have a list of properties of tool steels here. Please let me know which of the below mentioned properties will change after heat treatment?

16 August 2020 730 8 View

How to define hyper elastic UMAT in a UEL?

Hi all, I am working on Phase-Field UEL for hyperelastic materials. I am using codes of Dr Molnar ( http://www.molnar-research.com/tutorials_videos_3.html) to develop hyperelastic material. I...

07 April 2020 4,243 1 View

Related to Jasco FP-8300 spectrofluorometer?

I used Jasco FP-8300 spectrofluorometer to take a spectra of riboflavin but I got two instead of one. I had changed different solvent but the spectra remained same. Is this instrument fault or...

15 September 2019 633 1 View

What's going wrong with plastic sectioning?

I am doing Aniline blue staining in rice anthers to detect callose signaling. When anthers are squeezed and stained, I could detect the signaling. However, when I do plastic embedding with...

10 April 2019 9,131 15 View

What would be the formula for finding the stretch force for expanding an O ring made of elastic ? On what factors the expansion stretch force depends?

I want to expand an O ring made of elastic material and am looking for the mathematical expression (dynamics) on which the stretch force depends. In addition to this, how does it vary in different...

18 March 2019 4,158 3 View

Getting fewer colonies on SD-gal plates when compared to glu plates.Need help with increasing the transformation efficiency on gal plates?

Hello , I transformed yeast cells with a plasmid containing URA marker and plated on SD-Glucose and Galactose plates.I obtained about 350 colonies on the glucose plates but only 100 on galactose...

10 September 2017 9,431 8 View

Bacmid DNA is not getting dissolved in TE buffer

Hi.I am facing problems in the bacmid isolation step.Currently i am using purelink hipure plasmid miniprep kit for isolating the bacmid.But the problem is,after the isopropanol-ethanol step when i...

13 August 2017 1,069 10 View

What is a quick and dirty way to calculate dose response for a cancer drug on a cell line?

Besides MTT based assays what else are the available assays to evaluate drug dose for killing of cancer cell lines.

06 April 2017 1,626 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View