I'm interested in obtaining glucans fractions, mannans and glycoproteins from Phellinus.
If you were to use the fractions as test substances to living cells, try to optimize an aqueous extraction method to use.
I found these article that might provide you with some insights:
http://ieeexplore.ieee.org/document/5639487/?reload=true
Also, check this flowchart for you to have a general overview of metabolite extraction from plants and fungi:
https://patents.google.com/patent/EP2778177A1/en
I want to obtain the diketopiperazine fraction from a culture broth crude extract. The growth medium used was YMG.
07 August 2018 1,362 2 View
Could it be useful to determine the size of a fungal organism? Image for illustrative purposes only (Source: Webster & Weber 2007).
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I am searching for a useful alternative for NIST/EPA/NIH Mass Spectral Library.
03 April 2018 2,463 3 View
The free trial period for this software has expired and a license is currently out of reach.
03 April 2018 1,689 1 View
The protocol must be able to obtain DNA from bracket fungi.
03 April 2018 7,735 5 View
I want to examinate intraspecific variation in Phellinus sp. and its relationship with other members of the Hymenochaetaceae family.
03 April 2018 3,192 5 View
I'm seeking for a way to bypass the use of precision lab equipment as a way to raise the efficiency of field research.
02 March 2018 1,361 4 View
I want to compile some information about the leading universities or research centers in the field. Feel free to contribute.
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I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
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07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
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Palmitic acid presence in aqueous fraction
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I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View