Is there a standard method for extracting diketopiperazines from the culture broth of Hymenochaetoid fungi?

I want to obtain the diketopiperazine fraction from a culture broth crude extract. The growth medium used was YMG.

07 August 2018 1,286 2 View

Is there a viable open source/free alternative for the software CodonCode Aligner?

The free trial period for this software has expired and a license is currently out of reach.

03 April 2018 1,540 1 View

Is there a viable open source/free software for analyse and manage GC-MS spectra?

I am searching for a useful alternative for NIST/EPA/NIH Mass Spectral Library.

03 April 2018 2,349 3 View

What is the most efficient way for fungal DNA extraction?

The protocol must be able to obtain DNA from bracket fungi.

03 April 2018 7,658 5 View

Is vegetative incompatibility a good method to figure out if two strains of fungi are closely related?

Could it be useful to determine the size of a fungal organism? Image for illustrative purposes only (Source: Webster & Weber 2007).

03 April 2018 1,600 5 View

Is the internal transcribed spacer (ITS) region a good indicator of inter- and intraspecific variation in fungi?

I want to examinate intraspecific variation in Phellinus sp. and its relationship with other members of the Hymenochaetaceae family.

03 April 2018 3,113 5 View

Is there a simple or low-cost method for field-extracting VOCs from the fruiting body of bracket fungi?

I'm seeking for a way to bypass the use of precision lab equipment as a way to raise the efficiency of field research.

02 March 2018 1,283 4 View

Which are the world's best research institutions in fungal ecology and evolution?

I want to compile some information about the leading universities or research centers in the field. Feel free to contribute.

01 January 1970 2,368 18 View

No title

02 March 2021 3,060 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View