Is there a standard method for extracting diketopiperazines from the culture broth of Hymenochaetoid fungi?

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Joe Olechno

Cristian,

Most people extract the medium with a polar organic solvent like ethyl acetate or ethyl ether. See “Rhodotorulic acid, a diketopiperazine dihydroxamic acid with growth-factor activity. I. Isolation and characterization”, Curtis L. Atkin and J. B. Neilands, Biochemistry, 1968, 7 (10), pp 3734–3739 DOI: 10.1021/bi00850a054 or see the attached.

I hope that this helps.

Joe

Cristian Riquelme

Thanks Joe Olechno . Your answer was very helpful. Ethyl acetate seems to be the best option to start with. Regards.