Protocols for the production of silver nano particles from Pomegranate peel
You can cut peel into small pieces and incubate them in silver nitrate (you may chose concentrations ranging between 0.1 to 2 mM or even up to 5 mM). You can homogenize the peel in whatever solvent you wish and use supernatant after centrifugation (you may even use pellet) for generation of nanoparticles. You can boil the peel in double distilled water and take the extract (as well as peel) for generation of nanoparticles. You can use different volumes of the extract and also different concentrations of silver nitrate. Based on our experience, I strongly believe that all living systems and all their components (including biomolecules like sugars, phenolics, organic acids etc.) and even their dead biomass can generate Ag and Au nanoparticles. Hundreds of publications are available on use of plants or plant parts, microbes and their extracts and their biomass (including dead) [which include tea, coffee powder etc.) for generation of Ag and Au nanoparticles. Kindly note that extract of peel of pomegranate like several other plant/microbial extracts would have number of bio-molecules/bio-complexes that can generate nanoparticles and hence, in general we would get heterogeneous nanoparticles. It would be difficult to pinpoint which factor is largely responsible for reduction of silver ions and generation of Ag-nanoparticles. Therefore, I do not advise you to use something just based on the probable fact that no one worked with peal of pomegranate. Of course, if you have some concrete idea, please go ahead. It is just an advise and please do not consider this advice as discouragement. If you wish you may send any of your students to our lab, for training in generation of Ag and Au nanoparticles.
You may download our papers on generation of nanoparticles (including the papers where we showed role of phenolics, photosynthetic electron transport and dehydrogenases in nanoparticle synthesis).
hello sir Thank you so much for all your inputs and your precious time. Surely I will take you in a right sense when you say "based on the probable fact that no one worked with peal of pomegranate" I wrote this question since i have a student who wants to work on nano particles in her project and since in our lab we work on pomegranate peel we taught of doing nano particles and look at cytotoxic effect of it also with the same and since the student is very ambitious i just did not want to give false hopes to that and do something just for the sake of doing, since i am a very new to this area i wanted to take help from experienced people like you to get a correct idea for the same . lastly about the TRAINING you mentioned is really interesting Can i know more details about the same.
Number of students from various Universities come for learning few aspects of research or for carrying their M.Tch./M.Sc. dissertation work. Let me also make it very clear that we do not charge for such academic endeavours. If you wish, you may see our University website for details of all teachers profiles (which include e-mail addresses and phone numbers).
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