Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

Vacuum freeze drying protocol in human skeletal muscle tissue methods, anyone?

I will be freeze-drying small skeletal muscle samples (~60mg) per sample using a Virtis SP Scientific Benchtop Pro Freeze Dryer. I've had difficulty findings a reference protocol to determine the...

21 November 2020 1,423 1 View

Does anyone freeze samples in GeneJET RNA extraction kit lysis buffer?

For the GeneJET RNA purification kit from Thermo Scientific, does anyone know if you can lyse your sample (suspension cells in my case) in the lysis buffer from the kit and then keep the sample at...

18 October 2020 4,993 3 View

Looking for good/reliable betaine assay kits. Does anyone have experience with such a thing?

I am looking to quantify both human serum and skeletal muscle homogenate betaine concentrations via ELISA or colorimetric assay kits. Has anyone had a good experience with any products...

24 September 2020 4,550 3 View

Accurate curvature estimation of CT-scans (image stack)?

We have multiple CT-scans in the format of TIF image stacks. To get the mean curvature we first transform the stack to a triangular mesh (using Marching Cubes). To make the triangles in this mesh...

03 August 2020 7,921 4 View

How to calculate sample variance (and confidence intervals) for an effect size?

Hello, In relation to another recent, as yet unanswered, post (https://www.researchgate.net/post/How_to_calculate_comparable_effect_sizes_from_both_t-test_and_ANOVA_statistics), I am wondering...

18 June 2020 3,327 1 View

How to calculate comparable effect sizes from both t-test and ANOVA statistics?

Hello, I am currently undertaking a meta-analysis related to the efficacy of previous studies to harness pharmacological agents to ameliorate associative fear memories in rodents. The concept of...

15 June 2020 8,349 2 View

Input from experts in environmental survey sampling design on draft sampling design decision tree?

Hi. I am trying to help provide some rough advice for selecting amongst alternative sampling designs to measure bird density or abundance. The surveys are short-term in duration such as for impact...

22 January 2020 2,978 3 View

Test for silicone residue from silicone part?

I have a silicone part that will come in contact with food. It will need to pass FDA tests for hexane extraction of silicone residues. Is there a method I can use to remove uncured material and...

09 December 2019 4,246 3 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

How to troubleshoot Electrochromic Films and Electrochromic Devices?

I am trying to characterize an electrochromic system. The system uses ITO electrodes on glass, WO3 as the electrochromic film, and NiO as the ion storage layer. When I deposit NiO and WO3 and...

23 February 2021 9,592 2 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

How to improve the adhesion between germanium and silicon monoxide thin film layers using EBEAM evaporation?

I want to deposit multilayer coating of germanium and silicon monoxide on silicon wafer using ebeam evaporation?

21 February 2021 2,141 3 View

How can I measure the thickness of thin layers deposited on textured glass susbtrates?

I want to measure the thickness of aluminum oxide and titanium oxide layers deposited on textured glass substates. The layer thicknesses are typically below 100nm.

21 February 2021 5,465 4 View