I am studying the electrochemical behaviour of FeCl3 in the presence of a protein that forms a ligand with FeCl3. According to the reaction, with increasing concentration of the protein, more ligand binding should occur leading to decrease in Fe concentration which then gives reducing peak currents.
However, after a certain protein concentration, the peak current of FeCl3 is increasing to a value more than that obtained in the absence of protein.
What may be the reasons causing the rise?
Regards
If you use a detergent in your protein solution and you increase its concentration there is a risk to reach the critical micelle concentration.
Thank you sir but I haven't used any detergent solution. I have prepared the protein in NaCl buffer.
A protein denaturation is also possible with NaCl. Try to reduce the NaCl concentration. If the problem disappear or reappear, but at a higher protein concentration, you have a possible explanation and solution.
Try to read also about the salting out effect.
Thank you so much sir. I will definitely try reducing the NaCl concentration and observe the phenomena
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