What may be the possible reasons for getting only a single band during plasmid isolation?

12 January 2019 1 5K Report

Plasmids are always present in multiple forms. What might be the issue with my strain?

It can depend upon the plasmid and strain, it is possible to get a plasmid stock that is predominantly a single form, especially if you are using a RecA- strain that will stop formation of dimers and higher order recombination products.

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