Kumari Uma Mahto

12 Questions 13 Answers 0 Followers

Questions related from Kumari Uma Mahto

Where to perform invitro transcription and capping?

I am planning to do invitro transcription and capping for an mRNA vaccine generation. Can anyone suggest whether it should be done on a bench top or inside a laminar hood?

15 May 2024 274 3 View

What should be done if the modelled protein structure fails to pass VERIFY3D?

After refining the protein structure multiple times, it still fails to pass VERIFY3D. The ERRAT score is 96.9298 and PROCHECK results are fine. What can be done to improve the VERIFY3D results?

24 April 2023 6,802 3 View

What is the precise threshold concentration (in measurable units) of QS signaling molecules?

Bacterial Quorum Sensing relies on the synthesis, secretion, and detection of autoinducers to regulate gene expression in response to cell density. The small chemical signals are synthesized...

28 February 2023 2,786 0 View

What is the difference between hydraulic retention time and operating time in a wastewater treatment reactor?

Hydraulic retention time (HRT) is the average time a certain amount of wastewater stays in a holding tank before exiting the tank. But then what is the operating time?

20 January 2023 957 5 View

Problem in protein structure prediction using AlphaFold and subsequent docking using Autodock?

After the protein structure prediction using AlphaFold2, I want to proceed with molecular docking in Autodock. However, the modelled protein structure cannot be opened using Autodock with the...

04 January 2023 8,643 10 View

Can we evaluate EPS-protein and metal interaction using docking?

Biofilm bacteria show increased resistance to toxic heavy metals. The remarkable tolerance is often attributed to the EPS matrix. Several studies have confirmed the involvement of protein like...

18 February 2022 9,640 0 View

What is the difference between GC-MS and HRMS?

What is the difference between GC-MS and HRMS? Which is better for identification of compounds in a crude extract (mixture of compounds)?

13 July 2021 575 6 View

How to analyze biofilm images in COMSTAT?

I want to analyze the biofilm images obtained in CLSM through COMSTAT. I am using COMSTAT 2.1 Image J plugin. The workflow I am following is : 1. Import all the images in image J of a single...

04 March 2021 8,525 0 View

Will my revised and resubmitted paper be regarded as a new submission?

The editor commented that the manuscript in the current format is unsuitable for acceptance. However, the editor recommend to revise the manuscript in accordance with the reviewers comment and...

31 July 2020 6,654 4 View

How to submit whole genome sequence of bacteria?

During the whole genome sequence submission process of bacteria in NCBI, there comes a field "whether you want to annotate the genome using NCBI Prokaryotic Genome Annotation Pipeline (PGAP)". We...

21 December 2019 6,037 8 View

What may be the possible reasons for getting only a single band during plasmid isolation?

Plasmids are always present in multiple forms. What might be the issue with my strain?

12 January 2019 5,306 1 View

How can we measure corrosion due to biofilm formation?

Pseudomonas aeruginosa forms biofilm on different surfaces viz., glass, polystyrene, steel, ceramic and rubber. How can we quantify if the surfaces are being corroded after biofilm formation?

01 January 1970 7,703 5 View