What materials can I use to pin an anesthetized cockroach down without damaging or harming it during experimentation?

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Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

Is perfusion required to stain brain tissue with every method?

I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...

14 July 2024 177 4 View

Is there a validated questionnaire for target audience research?

Hi everyone, I am currently writing my masters thesis about Strategic Sponsor Acquisition in Youth Women's Soccer. The aim of the study is to identify potential sponsors for a specific youth...

04 July 2024 9,001 0 View

Posting a research survey ?

Hi i want to post this in various groups, how do i do that ? hey, please do my survey when you get the chance, it will take 15 minutes tops x I am looking for UK secondary school teachers to...

30 June 2024 9,594 2 View

Can I use richer media to increase bacterial expression of human protein?

We're using LB media, 1mM IPTG, and letting it incubate at 18°C over night. Will adding glucose or something else to the media increase bacterial expression?

06 June 2024 6,071 1 View

How to scale up or down a restriction enzyme reaction?

Hi I am trying to set up the reactions with my plasmid, insert and RE. Usually the standard final volume is 20 uL or 50 uL (it depends on the protocol provided by the manufacturer) with 1 ug of...

28 May 2024 1,105 3 View

Algae contamination on hydroponic cultures for Arabidopsis thaliana?

Hi everyone, I'm having trouble with my hydroponic cultures for Arabidopsis thaliana, since lately I've started having algae contamination on them. I have tried using several sterilization...

21 May 2024 4,840 1 View

How to set up a restriction enzyme reaction?

I'm a doctoral student with early experiences with recombinant protein synthesis. Since I still have to start my experiment and I do not yet know the quantities of DNA and plasmid that I will...

19 May 2024 5,171 3 View

What is recommended as a strong and current reference of phenomenological analysis ?

What is recommended as a strong and current reference of qualitative phenomenological analysis ?

16 May 2024 2,142 1 View

Is there any guide to perform statistical analysis of transcriptomics?

I am looking for a some chapter or book related to the statistical analysis of mRNA transcriptomics. Thank you;)

09 May 2024 6,543 2 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

Publishing a checklist?

Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...

01 August 2024 6,134 2 View

How can I handle unavailable papers in a systematic literature review?

Context: I am writing a Systematic Literature Review (SLR) as part of my master's thesis project. After initially screening the titles and abstracts of 338 papers from four selected databases, I...

29 July 2024 9,370 4 View

China (Qinghai) flowers identification?

Can someone help me in identification of some flowers observed in Tibetan plateau (Qinghai province)? See the attached pdf. Sincerely Armando

18 July 2024 5,125 4 View

What are the key risks associated with foodborne pathogens in imported leafy greens, the impact on public health and effect on regulatory framework?

I am currently writing a systematic review

15 July 2024 9,176 1 View

¿Should a pilot study be done before conducting a systematic review?

14 July 2024 3,036 3 View

Can ROBINS-I be used to measure risk of bias in single-arm studies?

I am doing a systematic review, and I am measuring risk of bias with RoB2 for RCT, and ROBINS-I for non RCT. My questions is, for single arm studies, can I use ROBINS-I? I am not sure how to...

14 July 2024 3,570 5 View

How can I find a subject for PhD?

I need help for find a subject for PhD in insect physiology

12 July 2024 8,227 1 View

Niklas Beckers

I would suggest liquid wood glue (the white stuff) on paper. Takes only a few seconds to harden, can be easily removed with water afterwards

Rebekah Cioffi

I used really sticky white Blu Tack to stick down diving beetles while I encapsulated them, however their external cuticle must be dry first, otherwise they just wriggle themselves loose.

Ramesh Chandra Tripathi

you can use transparent sticky fluid