What kind of sodium bicarbonate is recommended as a vehicle for mice?

11 November 2013 0 9K Report

Hi, I'm starting to do some research with mice (C57BL6J). I need to inject folic acid in 0.3M sodium bicarbonate (intraperitoneal). Does anyone know which type of sodium bicarbonate is recommended?

Badges
Science topic

More Elena Guillén's questions See All

Can room temperature affect Western-blot?

Hello, everyone, I've been doing Western-blocks for years. For a few days now I have had problems, even with "easy" antibodies such as GAPDH. According to my findings, proteins are in good...

05 June 2018 2,026 4 View

Can you help me with unexpected band in western-blot?

Hello and thank you,I used Allprotect Tissue Reagent to stabilize human kidney samples. After some months I extracted DNA, RNA and protein with the AllPrep DNA/RNA/Protein Mini Kit from Qiagen....

06 July 2015 6,080 16 View

Does anyone have knowledge on the disruption of kidney tissue with a bed mill homogenizer?

Hi, I need to do RNA and protein extraction of many kidney samples that are frozen at -80ºC in AllProtect reagent. I would like to use a multiple sample homogenizer like Tissuelyser LT, Bullet...

09 October 2014 4,134 1 View

Molecular weight marker for non-reduced proteins

I'm doing some western blots with a large protein (180 kDa) under non-reduced conditions. The band I observed in the western is about 220kDa. Does anyone know if the molecular weights from my...

10 November 2012 1,806 11 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

Why can't academics earn the money they deserve?

Only Journals make money from the articles we have worked on for years. Academics do not earn money from their refereeing. Then shouldn't the solution be a system in which academics can earn...

01 August 2024 6,469 6 View

Posthoc test lettering in JAMOVI?

Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...

31 July 2024 3,333 4 View

Where to download DEM from 2018?

I am currently working on validating land subsidence results obtained using SBAS InSAR. Could anyone suggest reliable sources or repositories where I can download a historical DEM from 2018?...

31 July 2024 9,757 3 View