Hi, I'm starting to do some research with mice (C57BL6J). I need to inject folic acid in 0.3M sodium bicarbonate (intraperitoneal). Does anyone know which type of sodium bicarbonate is recommended?
Hello, everyone, I've been doing Western-blocks for years. For a few days now I have had problems, even with "easy" antibodies such as GAPDH. According to my findings, proteins are in good...
05 June 2018 1,969 4 View
06 July 2015 5,995 16 View
09 October 2014 4,094 1 View
I'm doing some western blots with a large protein (180 kDa) under non-reduced conditions. The band I observed in the western is about 220kDa. Does anyone know if the molecular weights from my...
10 November 2012 1,746 11 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...
02 March 2021 6,865 1 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....
28 February 2021 7,967 3 View
The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...
27 February 2021 6,350 2 View
In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...
25 February 2021 5,229 3 View
I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.
22 February 2021 7,800 3 View
Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...
21 February 2021 7,260 4 View
Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...
21 February 2021 8,132 1 View
I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...
18 February 2021 6,896 3 View