Elena Guillén

5 Questions 16 Answers 0 Followers

Questions related from Elena Guillén

Can room temperature affect Western-blot?

Hello, everyone, I've been doing Western-blocks for years. For a few days now I have had problems, even with "easy" antibodies such as GAPDH. According to my findings, proteins are in good...

06 June 2018 2,007 4 View

Can you help me with unexpected band in western-blot?

Hello and thank you,I used Allprotect Tissue Reagent to stabilize human kidney samples. After some months I extracted DNA, RNA and protein with the AllPrep DNA/RNA/Protein Mini Kit from Qiagen....

07 July 2015 6,055 16 View

Does anyone have knowledge on the disruption of kidney tissue with a bed mill homogenizer?

Hi, I need to do RNA and protein extraction of many kidney samples that are frozen at -80ºC in AllProtect reagent. I would like to use a multiple sample homogenizer like Tissuelyser LT, Bullet...

10 October 2014 4,119 1 View

What kind of sodium bicarbonate is recommended as a vehicle for mice?

Hi, I'm starting to do some research with mice (C57BL6J). I need to inject folic acid in 0.3M sodium bicarbonate (intraperitoneal). Does anyone know which type of sodium bicarbonate is recommended?

11 November 2013 8,902 0 View

Molecular weight marker for non-reduced proteins

I'm doing some western blots with a large protein (180 kDa) under non-reduced conditions. The band I observed in the western is about 220kDa. Does anyone know if the molecular weights from my...

11 November 2012 1,783 11 View