5 Questions 16 Answers 0 Followers
Questions related from Elena Guillén
Hello, everyone, I've been doing Western-blocks for years. For a few days now I have had problems, even with "easy" antibodies such as GAPDH. According to my findings, proteins are in good...
06 June 2018 2,006 4 View
Hello and thank you,I used Allprotect Tissue Reagent to stabilize human kidney samples. After some months I extracted DNA, RNA and protein with the AllPrep DNA/RNA/Protein Mini Kit from Qiagen....
07 July 2015 6,052 16 View
Hi, I need to do RNA and protein extraction of many kidney samples that are frozen at -80ºC in AllProtect reagent. I would like to use a multiple sample homogenizer like Tissuelyser LT, Bullet...
10 October 2014 4,118 1 View
Hi, I'm starting to do some research with mice (C57BL6J). I need to inject folic acid in 0.3M sodium bicarbonate (intraperitoneal). Does anyone know which type of sodium bicarbonate is recommended?
11 November 2013 8,901 0 View
I'm doing some western blots with a large protein (180 kDa) under non-reduced conditions. The band I observed in the western is about 220kDa. Does anyone know if the molecular weights from my...
11 November 2012 1,781 11 View