Any non-OPA HPLC method for amino acids analysis?

I'm planing to analyze amino acids by HPLC. However, OPA-based method is complicated and does not reach the safety policy. Therefore, is there any other HPLC method for amino acids analysis?...

05 June 2018 6,297 10 View

Which is the best way to sterilize a very low pH solution (pH 1.5), autoclave or filtration?

I need to prepare PBS buffer which has very low pH (pH = 1.5). I'm considering filtration method. Does a very low pH solution interacts with filter member? Thank you.

05 June 2018 8,784 4 View

How to minimize a noise background from Sanger sequencing?

I'm working on Sanger sequencing using Bigdye V.3.1 and purify the sequenced product by DyeEx 2.0 kit. The chromatogram was fine and readable, however, it always contains some noise background and...

10 November 2017 9,673 10 View

How to extract the crude enzyme from yeast culture?

I have studied about the mitochondrial enzyme in yeast. So, I want to extract the crude enzyme from cell culture in order to test the enzyme activity. What is the suitable procedure in this case?...

08 September 2016 6,015 3 View

Protein expression in E.coli, located in inclusion-body, how to purify without losing an enzymatic activity?

07 August 2016 766 9 View

I am stuck with a high Tm primer for PCR? Why do I get no PCR product?

31 December 2015 6,655 14 View

What is the suitable plasmid or selective media for S.cerevisiae S288c transformation?

I need to transform pRS416 plasmid containing an interesting point mutation gene to S288c which contained random mutation. Since this strain can grow on SD media so I've just wondered, what media...

01 February 2015 5,198 1 View

How can I verify yeast transformed with point mutation plasmid?

I have introduced 1 base mutation which cause 1 amino acid changed by PCR then transformed this plasmid to DH5-alpha. At this step, I checked desire transformants byspread on selective media...

11 December 2014 8,237 4 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

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25 February 2021 5,713 1 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 2,968 1 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View