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Questions related from Natthaporn Takpho
I don't have any background in bioinformatics just had a basic biostatistic course back in university. If I need to use the package platform like LEfSE to screen for potential biomarkers from...
08 August 2018 3,957 1 View
I need to prepare PBS buffer which has very low pH (pH = 1.5). I'm considering filtration method. Does a very low pH solution interacts with filter member? Thank you.
06 June 2018 8,846 4 View
I'm planing to analyze amino acids by HPLC. However, OPA-based method is complicated and does not reach the safety policy. Therefore, is there any other HPLC method for amino acids analysis?...
06 June 2018 6,363 10 View
I'm working on Sanger sequencing using Bigdye V.3.1 and purify the sequenced product by DyeEx 2.0 kit. The chromatogram was fine and readable, however, it always contains some noise background and...
11 November 2017 9,737 10 View
I have studied about the mitochondrial enzyme in yeast. So, I want to extract the crude enzyme from cell culture in order to test the enzyme activity. What is the suitable procedure in this case?...
09 September 2016 6,075 3 View
I constructed a pET-53 to express yeast gene in E. coli BL21(DE3). The culture condition was culture the BL21(DE3) until OD600 = 0.4
08 August 2016 846 9 View
I'm doing PCR with primer tagging with attB1 and attB2 sequences for gateway cloning system. My target gene was ligated to pRS416 plasmid, 2 kb and 1 kb in sized. I'm using KOD FxNeo PCR kit and...
01 January 2016 6,707 14 View
I need to transform pRS416 plasmid containing an interesting point mutation gene to S288c which contained random mutation. Since this strain can grow on SD media so I've just wondered, what media...
02 February 2015 5,246 1 View
I have introduced 1 base mutation which cause 1 amino acid changed by PCR then transformed this plasmid to DH5-alpha. At this step, I checked desire transformants byspread on selective media...
12 December 2014 8,295 4 View
