Hi all,
I've been encountered some contamination in my time, but this time I am truly puzzled. I am working with non-adherent glioblastoma cells and recently I observed the cells starting to form these weird structures that float on the supernatant and don't break the water-tension (see pictures).
When removed (during splitting), they reappear within a few days and can reach to 0.5 cm in radius. Did anyone see this before and can identify this?
The structure is branching and is reminiscent of a fungus, but it is unlike a fungus I have ever seen in cell culture, non-toxic to the cells. Adding Fungicides like Fungin (Invivogen) appears to make no difference.
I would appreciate any help or suggestions :)
I added 200 and 300% of the proposed concentration and this seems to make the growth disappear, so it is likely to be fungi after all. Apparently, adding insufficient fungicide puts the fungi under stress and enhances the growth to a certain point. Adding more does the trick :)
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