What isthe difference between detecting the mrna expression and the translated corresponding protein ?

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Adam B Shapiro

It has been found that mRNA levels are not well-correlated with protein levels. Therefore, it is best to measure directly the parameter of interest.

Eman G Ayad

Thank you it was helpful

Andreas Friedrich

I agree with Adam, you can also have a look at the Schwanhäusser Paper from 2011, they have a very good explaination on that topic.

Sivadas VP

Detecting/quantifying the target protein is the confirmatory step for the activity/effect of your drug. But, it also depends on whether you have the facilities and expertise to conduct protein level studies. mRNA level studies are much easier to conduct, as compared to protein level studies.

In many cases, it is possible to perform an ELISA to measure the amount of the protein thanks to the commercial availability of antibodies and sometimes whole kits.