I have used RAPD for profiling and got little or no bands, now I want to try ISSR primers. So I am seeking to know if anybody has done similar work and the primers used.
Hi! How many RAPD primers have you tried! RAPDs are very sensitive. They will not work in the first setting itself. There will be more primers - 'n' number of primers. Some will give good amplification some hardly give bands. Omit them, first make trail by taking one sample and many primers (how many you can check). Select from that which gave good number of bands. Try with all samples. Once you complete RAPD use ISSR also you have both data. Check the attached article.
Good Luck!!
Around 2011 & 12 I answered many questions related to RAPD PCR problems. If you have patience you can check in my answers link. Use Ta 35C it works fine. Try and let me know the results.
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