What is your operational definition of induced latency?
I have repeated an MTT assay three times using different batches of the same cell line. Each time, I tested the same treatment (different medium) and seeded the same number of cells. Additionally,...
08 July 2024 1,900 1 View
Good day! I am the CEO of an online magazine called Order Of The Pen . We specialize in promoting new writers via our platform on Wix and Partnership with Mailchimp for email marketing,...
18 June 2024 4,117 0 View
Hi, I am attempting to culture 3T3-F442A cells and carry out IF staining. I'm testing a variety of different compounds on the cells (e.g. lopinavir, clozapine, olanzapine etc.) Up until recently,...
16 June 2024 2,754 0 View
Dear network I need to plot instantaneous and mean End-to-end delay in D2D and eNode-based topologies? How to generate/visualise the accurate vec file data? I appreciate your help
14 May 2024 2,897 1 View
I'm planning a small research project involving conditioned medium from cancer cells on a human cell line (specifically KGN cells). I intend to expose the cancer cells to this medium for 72 hours...
06 May 2024 8,470 2 View
I'm considering submitting a manuscript to this journal (Tobacco Regulatory Science ISSN: 2333-9748 ) Has anyone published in this journal before? I am looking to confirm that this journal is...
05 March 2024 4,144 1 View
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07 January 2024 8,745 1 View
Ray is a unified framework for scaling AI and Python applications. Ray consists of a core distributed runtime and a set of AI Libraries for accelerating ML workloads.
23 December 2023 6,831 3 View
Supervised Learning In supervised learning, the dataset is labeled, meaning each input has an associated output or target variable. For instance, if you're working on a classification problem to...
11 December 2023 1,655 5 View
Hello, I am currently performing DNA extraction from an anaerobic digester using the FastDNA spin kit for soil, which involves 2 SEWS-M wash steps. Prior to extraction, I subjected the samples to...
23 October 2023 2,790 0 View
I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...
30 June 2024 6,502 5 View
I am a first-time researcher in the field of nursing and i would like to conduct research about knowledge and attitude towards prevention and spread of tuberculosis.
11 June 2024 3,821 2 View
In 2024, childhood tuberculosis will still be a significant global public health issue, especially in underdeveloped nations (LMICS). It is true that the underestimation of the disease's extent...
31 May 2024 5,221 0 View
Hello, I have propagated a plasmid form a single colony (size around 15 kb) using small scale culture (2 ml of TB with selected Antibiotic) and purified it using Mini-prep kits ( FastGene Plasmid...
14 May 2024 6,123 2 View
We are standardizing chromatographic conditions for first-line TB drugs in our newly procured UHPLC system. For Ethambutol, we are not able to get dedicated chromatogram. Can anyone suggest any...
24 April 2024 9,859 0 View
Hello, everyone. This is Dr Prerna, SR Biochemistry, NITRD, New Delhi. We are developing methods for Therapeutic drug monitoring of first line TB drugs in our laboratory. After utilizing suitable...
17 April 2024 9,598 0 View
The pan-country study could have further divisions/categorization based on different criteria. Any recommendations would be greatly appreciated. Thank you!
27 March 2024 991 1 View
I am interested in the study of dendritic cells response under infection with mutant strain candidate to vaccine against TB. But I am begginer in the work with dendritic cells, somebody can help...
20 March 2024 1,587 7 View
I am doing a resazurin assay to determine the MIC of plant extracts against Mycobacterium tuberculosis. The problem is the MIC is coming at a very high value. I read in one paper that even if...
01 January 2024 7,916 2 View
If Iqpt/Q = 1 and the number of airborne quanta inhaled by susceptibles equaled the number of susceptibles, some susceptibles would escape infection and others would inhale more than one quantum....
24 December 2023 1,144 0 View