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Dear Ms Tigist Bekele,

1. You have to filter the oil red O stain material

2. Please notice that many fat globules, compine with the stain, and flow over the surface of the solution. You have to concentrate your study into the cell not over the floating items (it is normal to see many fat globules and some stain on the surface of the examined tissue).

3. Another option is to use Sudan III, and keep in mind to avoid artefact from the cellular debris and stain impurties.

Best wishes

Thank you so much Prof. Mamdouh Afify.

Hi , how to do calculate lipid content in hepg2 cells with red oil beside visualization ?

Dear Melika Ameli,

Using Oil Red O to calculate the lipid content would be helpful if you use an image program and be aware of the artefacts. Compination of both would be better. In addition, if possible use an biochemical analytical method.

Best wishes

Hello Melika,

You can use nile red, instead of oil red, since its more sensitive for intracellular lipid detection.

Regards

Richa