I would like to study the interaction between cassava viruses and a sort of cassava genotypes. I would like to do this by measuring virus concentration. Is it right to call this virus titer/concentration? I am working with RNA viruses.
Hi,
Titer is expressed as
-infectious unit/mL.
-viral protein pg/mL (p24 core protein for HIV-1),
-RNA copies/mL
Hi, read some literature, f.g. this:
http://www.sciencedirect.com/science/article/pii/S0166093401003160
http://www2.oakland.edu/biology/chaudhry/pics/virologicalmethods.pdf
Good luck!
Cheers, Nadine
Hi,
Titer is expressed as
-infectious unit/mL.
-viral protein pg/mL (p24 core protein for HIV-1),
-RNA copies/mL
You can measure the titer in cell culture or in lab animals but the concentration of viral antigens by ELISA etc, concentration of the RNA by Real-Time PCR. It depends what you need for your research...
If you try to study the effect of virus in different hosts genotypes, real-time PCR is more indicated, because you can distinguish small differences in replication and quantify these. I guess, this is a central question in your work ..Good luck!
For doing the viral titre assay............ Plaque forming unit assay or Focus forming unit assay would be a good choice as it gives the count of live infecting particles rather than getting a count of nuclelc acid level from RT PCR which can be seen in non-infectious particles too.
To complete previous response you can also mesure the viral titer by TCID50 method (on vero cells for exemple) if you can detect a CPE with your virus.
The advantage of viral titer by PFU or TCID50 is to quantify replicative virus.
The nombres of infectieux virus forme in volume of solution .
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